diff --git a/src/tests/Loader/classloader/generics/ByRefLike/Validate.csproj b/src/tests/Loader/classloader/generics/ByRefLike/Validate.csproj
index bab89d738420b3..ab734484816313 100644
--- a/src/tests/Loader/classloader/generics/ByRefLike/Validate.csproj
+++ b/src/tests/Loader/classloader/generics/ByRefLike/Validate.csproj
@@ -1,4 +1,7 @@
 <Project Sdk="Microsoft.NET.Sdk">
+  <PropertyGroup>
+    <MonoAotIncompatible>true</MonoAotIncompatible>  <!-- https://github.com/dotnet/runtime/issues/90427  -->
+  </PropertyGroup>
   <ItemGroup>
     <Compile Include="Validate.cs" />
     <Compile Include="GenericTypeSubstitution.cs" />